Walski M, Owczarska K, Borowicz J
Laboratory of the Ultrastructure of the Nervous System, Polish Academy of Sciences, Warsaw.
J Hirnforsch. 1995;36(3):399-410.
The study was concerned with the nuclei of neurons derived from the cerebral cortex of rats that underwent experimental cardiac arrest for a duration of 10 minutes and then, after reanimation, survived from 2 weeks up to 10 months. The animals were divided into two groups: in the first-those specimens that survived from 2 to 6 weeks; in the second-those that survived 8 weeks to 10 months. In the specimens obtained from the first group we observed irregular vesicular structures and bundles of parallel fibrils 8 nm in diameter. In the second group we often observed paracrystalline form resembling "plaited ropes" and tubulous structures linked by a microfibrillar netting. These structures were analysed with a gonyometer. We believe that both the paracrystalline and the irregular vesicular structures that have been observed in the neurons obtained from ischaemic brains appeared due to the alterations in the structure of the karyoskeleton and that the observed intracellular structures are not specific for post-ischaemic alterations.
该研究关注的是源自经历了10分钟实验性心脏骤停、复苏后存活2周直至10个月的大鼠大脑皮层的神经元细胞核。这些动物被分为两组：第一组是存活2至6周的标本；第二组是存活8周至10个月的标本。在从第一组获得的标本中，我们观察到不规则的囊泡结构和平行排列的直径为8纳米的纤维束。在第二组中，我们经常观察到类似“辫绳”的准晶体形式以及由微纤维网连接的管状结构。用测角仪对这些结构进行了分析。我们认为，在缺血性脑获得的神经元中观察到的准晶体结构和不规则囊泡结构都是由于核骨架结构的改变而出现的，并且观察到的细胞内结构并非缺血后改变所特有的。
