Sakamoto H
Department of Orthopedic Surgery, Akita University School of Medicine, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1995 Jul;69(7):484-92.
The solubility of calcium pyrophosphate dihydrate crystals (CPPD crystals), which cause pseudogout, was studied in vitro and vivo. The in vitro experiment using 0.1 M tris buffer and 0.2 M glycine buffer indicated that changes in pH and pyrophosphatase activity played a major role in the solubility of CPPD crystals. An experiment using the synovial fluid from patients with pseudogout, rheumatoid arthritis, and from those with osteoarthritis suggested that changes in the synovial fluid pH due to inflammation affected the solubility of these crystals. In addition, an experiment using the air pouch in rat showed that inflammation due to the CPPD crystals was maximum at about 9 hours after CPPD injection, and that inflammatory cells appearing at this time then had a major influence on the crystals' solubility. From these results, it appeared that CPPD crystals released into the joint cavity were mostly dissolved by inflammatory cells, but that crystal dissolution was also affected by changes in the synovial fluid itself, particularly by a change in pH.
对引起假痛风的二水焦磷酸钙晶体（CPPD晶体）的溶解度进行了体外和体内研究。使用0.1M三羟甲基氨基甲烷缓冲液和0.2M甘氨酸缓冲液的体外实验表明，pH值和焦磷酸酶活性的变化在CPPD晶体的溶解度中起主要作用。一项使用假痛风患者、类风湿性关节炎患者以及骨关节炎患者的滑液进行的实验表明，炎症引起的滑液pH值变化会影响这些晶体的溶解度。此外，一项使用大鼠气袋的实验表明，CPPD晶体注射后约9小时，由CPPD晶体引起的炎症最为严重，此时出现的炎症细胞随后对晶体的溶解度有重大影响。从这些结果来看，似乎释放到关节腔中的CPPD晶体大多被炎症细胞溶解，但晶体溶解也受到滑液本身变化的影响，特别是pH值的变化。
