Chan S Y, Pearlstone A, Uhler M, Tucker M, Greenspoon R, Leung A, Wang C
Division of Reproductive Endocrinology and Infertility, Cedars-Sinai Medical Center, Los Angeles, CA 90048.
Hum Reprod. 1993 May;8(5):717-21. doi: 10.1093/oxfordjournals.humrep.a138127.
Normozoospermic semen samples (n = 82) were examined to investigate whether the degree of sperm tail swelling in hypo-osmotic medium (fructose and sodium citrate; 150 mOsm/l), and motility characteristics after a 15-min exposure to hypotonic saline (sodium chloride; 150 mOsm/l) could predict the cryosurvival rate (% post-thaw motility/% pre-freeze motility x 100%) of spermatozoa after cryopreservation by the liquid nitrogen vapour freezing method using the TEST-glycerol-egg yolk buffer. The CellSoft automated semen analyser was used to analyse sperm motility in pre-freeze and post-thaw semen samples, and after exposure to hypotonic saline. Sperm tail hypo-osmotic swelling and sperm motility in pre-freeze semen showed no significant correlations (P > 0.05) with the cryosurvival rate. There were significant correlations (P < 0.05) between the cryosurvival rate and the following sperm motility parameters in hypotonic saline: % motility (r = 0.2846), motility index (% motility x curvilinear velocity; r = 0.2809) and % decrease in motility index from the baseline value in semen (r = 0.3378). The % decrease in motility index after hypotonic saline treatment was significantly less (P < 0.05) in the normal (> or = 50% cryosurvival rate; mean +/- SEM 5.9 +/- 3.2%; n = 33) compared with the subnormal (< 50% cryosurvival rate; 27.3 +/- 4.8%; n = 49) cryosurvival groups. This parameter was also determined, by multivariate discriminant analysis, to be capable of classifying each pre-freeze semen sample into either cryosurvival group with 69.5% accuracy.(ABSTRACT TRUNCATED AT 250 WORDS)
对正常精子精液样本（n = 82）进行检测，以研究低渗介质（果糖和柠檬酸钠；150 mOsm/l）中精子尾部肿胀程度以及暴露于低渗盐水（氯化钠；150 mOsm/l）15分钟后的运动特性，能否预测使用TEST-甘油-蛋黄缓冲液通过液氮蒸汽冷冻法冷冻保存后精子的冷冻存活率（解冻后活力/%冷冻前活力×100%）。使用CellSoft自动精液分析仪分析冷冻前和解冻后精液样本以及暴露于低渗盐水后的精子活力。冷冻前精液中精子尾部低渗肿胀和精子活力与冷冻存活率无显著相关性（P > 0.05）。低渗盐水中的冷冻存活率与以下精子运动参数存在显著相关性（P < 0.05）：活力百分比（r = 0.2846）、活力指数（活力百分比×曲线速度；r = 0.2809）以及与精液中基线值相比活力指数的下降百分比（r = 0.3378）。与冷冻存活率低于正常水平（< 50%；27.3 ± 4.8%；n = 49）的组相比，正常冷冻存活率（>或 = 50%；平均±标准误5.9 ± 3.2%；n = 33）的组在低渗盐水处理后活力指数的下降百分比显著更低（P < 0.05）。通过多变量判别分析确定，该参数能够以69.5%的准确率将每个冷冻前精液样本分类到相应的冷冻存活组中。（摘要截断于250字）
