Mulay S, Conliffe P R, Varma D R
Department of Medicine, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada.
J Endocrinol. 1995 Aug;146(2):255-9. doi: 10.1677/joe.0.1460255.
The main purpose of these studies was to determine whether diabetic pregnancy altered maternal and fetal atrial natriuretic peptide (ANP). Diabetes was induced in rats by intravenous injection of 40 mg streptozotocin/kg on day 2 of gestation. Immunoreactive ANP in plasma, amniotic fluid and hearts on day 20 of gestation was measured by radioimmunoassay; fetal cardiac natriuretic peptides (ANP, proANP and BNP) were separated by reverse-phase high pressure liquid chromatography. Diabetes caused an increase in fetal plasma insulin, placental weight, amniotic fluid volume, the ratio of the fetal heart to body weight, maternal and fetal plasma ANP, fetal cardiac ANP and fetal cardiac BNP. It is suggested that the maternal diabetes-induced increase in fetal ANP might be related to fetal myocardial hypertrophy and could contribute to hydramnios.
这些研究的主要目的是确定糖尿病妊娠是否会改变母体和胎儿的心房利钠肽（ANP）。在妊娠第2天通过静脉注射40mg链脲佐菌素/ kg诱导大鼠患糖尿病。通过放射免疫测定法测量妊娠第20天时血浆、羊水和心脏中的免疫反应性ANP；通过反相高压液相色谱法分离胎儿心脏利钠肽（ANP、proANP和BNP）。糖尿病导致胎儿血浆胰岛素增加、胎盘重量增加、羊水量增加、胎儿心脏与体重之比增加、母体和胎儿血浆ANP增加、胎儿心脏ANP和胎儿心脏BNP增加。提示母体糖尿病引起的胎儿ANP增加可能与胎儿心肌肥大有关，并可能导致羊水过多。
