van Engelenburg F A, Kaashoek M J, van Oirschot J T, Rijsewijk F A
Open Department of Virology, Institute for Animal Science and Health, Lelystad, The Netherlands.
J Gen Virol. 1995 Sep;76 ( Pt 9):2387-92. doi: 10.1099/0022-1317-76-9-2387.
To gain insight into the role of glycoprotein E of bovine herpesvirus 1 (BHV-1), we compared the distribution of wild-type (wt) BHV-1 with that of a gE deletion mutant (gE-) in calves after intranasal inoculation. The wt-infected calves had severe clinical signs, but the gE(-)-infected calves were virtually free of clinical signs. At 3, 4, 7, 8, 44, 45, 50 and 51 days post-infection (p.i.), one calf from each group was killed and tissues were collected for virus isolation and PCR analysis. At 3, 4, 7 and 8 days p.i., infectious virus could be isolated only from the nasopharyngeal mucosa, parotid gland and nearby lymphoid tissues for both the wt- and gE(-)-infected calves. At 3 and 4 days p.i., virus titres in these tissues were comparable in both the wt- and gE(-)-infected calves. However, the virus titres were significantly reduced at 7 and 8 days p.i. in the gE(-)-infected calves, but not in the wt-infected calves. Semi-quantitative PCR analysis revealed that for the entire infection period (3 to 51 days p.i.) significantly more BHV-1 DNA was detected in the trigeminal ganglia (TG) of the wt-infected calves than in those of the gE(-)-infected calves. We conclude that the gE- mutant infects the same limited number of tissues as wt BHV-1, but for a shorter period.
为深入了解牛疱疹病毒1型（BHV-1）糖蛋白E的作用，我们比较了鼻内接种后野生型（wt）BHV-1和gE缺失突变体（gE-）在犊牛体内的分布情况。wt感染的犊牛出现严重临床症状，但gE（-）感染的犊牛几乎没有临床症状。在感染后（p.i.）3、4、7、8、44、45、50和51天，每组处死一头犊牛并采集组织用于病毒分离和PCR分析。在感染后3、4、7和8天，wt和gE（-）感染的犊牛均仅能从鼻咽黏膜、腮腺及附近淋巴组织中分离到感染性病毒。在感染后3和4天，wt和gE（-）感染的犊牛这些组织中的病毒滴度相当。然而，在感染后7和8天，gE（-）感染的犊牛病毒滴度显著降低，而wt感染的犊牛则未降低。半定量PCR分析显示，在整个感染期（感染后3至51天），wt感染的犊牛三叉神经节（TG）中检测到的BHV-1 DNA显著多于gE（-）感染的犊牛。我们得出结论，gE突变体与wt BHV-1感染相同数量有限的组织，但感染时间较短。
