Giamarellos-Bourboulis E J, Grecka P, Giamarellou H
1st Department of Propedeutic Medicine, Laiko General Hospital, University of Athens, Medical School, Greece.
J Chemother. 1995 Jun;7(3):179-83. doi: 10.1179/joc.1995.7.3.179.
In order to compare the in vitro killing activity of meropenem and imipenem against multiresistant P.aeruginosa 14 strains were used. All nosocomial isolates were susceptible to meropenem and imipenem minimum inhibitory concentration (MIC < or = 4 micrograms/ml) and resistant to at least two other antimicrobial agents of diverse chemical class with antipseudomonal activity. Forty-two killing curves were performed by exposing a 5 x 10(5) CFU/ml log-phase inoculum to 1x minimum bactericidal concentration (MBC) of each carbapenem. Meropenem was found to possess a slower killing rate than imipenem over the first 5 hours of P.aeruginosa exposure, but to be equally effective as imipenem after 24 hours of incubation. Forty percent and 11.1% of P.aeruginosa strains developed resistance to imipenem and meropenem respectively after a 24-hour exposure to carbapenem. The authors speculate about the underlying mechanisms explaining the higher rate of resistance development to imipenem than to meropenem.
为比较美罗培南和亚胺培南对多重耐药铜绿假单胞菌的体外杀菌活性，使用了14株菌株。所有医院分离株对美罗培南和亚胺培南敏感（最低抑菌浓度MIC≤4微克/毫升），并对至少两种具有抗假单胞菌活性的不同化学类别的其他抗菌药物耐药。通过将5×10⁵CFU/毫升对数期接种物暴露于每种碳青霉烯类药物的1倍最低杀菌浓度（MBC），进行了42次杀菌曲线实验。发现在暴露于铜绿假单胞菌的前5小时内，美罗培南的杀菌速率比亚胺培南慢，但在孵育24小时后与亚胺培南同样有效。在暴露于碳青霉烯类药物24小时后，分别有40%和11.1%的铜绿假单胞菌菌株对亚胺培南和美罗培南产生耐药性。作者推测了解释亚胺培南耐药性发展速率高于美罗培南的潜在机制。
