Alvares O, Klebe R, Grant G, Cochran D L
Department of Periodontics, University of Texas Health Science Center, San Antonio, USA.
J Periodontol. 1995 Jul;66(7):552-8. doi: 10.1902/jop.1995.66.7.552.
The fibroblast is a prominent cellular component of the periodontal ligament. It is believed to play an important role in collagen metabolism in health and disease. The turnover of collagen in the periodontal ligament is believed to be controlled by the balance between collagen synthesis and degradation. The family of matrix metalloproteinases and their inhibitors is one of the mechanisms which regulates this balance. The factors that regulate the synthesis of collagenase and its inhibitor, TIMP-1, by the periodontal ligament cell are poorly understood. The present study was undertaken to assess the effect of interleukin-1 beta (IL-1 beta), platelet-derived growth factor (PDGF), and transforming growth factor-beta 1 (TGF-beta) on the expression of collagenase (MMP-1) and TIMP-1 mRNA in periodontal derived fibroblasts using reverse transcription polymerase chain reaction (RT-PCR). Early passage periodontal ligament derived fibroblasts were treated with IL-1 beta (10 and 100 pg/ml), two isoforms of PDGF, -AA and -BB (4 and 20 ng/ml) and TGF-beta (1 and 10 ng/ml). Treatment with growth factors from 2 to 24 hours revealed that the largest effects on MMP-1 mRNA occurred after 24 hours. IL-1 beta induced a 5 to 9 fold increase in MMP-1 mRNA. The two isoforms of PDGF had less of an effect (3 to 5 fold) on MMP-1 mRNA whereas TGF-beta induced a 25 to 50% decrease in the expression of this message. None of the growth factors had an effect on TIMP-1 mRNA expression.(ABSTRACT TRUNCATED AT 250 WORDS)
成纤维细胞是牙周膜中一种重要的细胞成分。据信它在健康和疾病状态下的胶原代谢中发挥重要作用。牙周膜中胶原的周转被认为受胶原合成与降解之间平衡的控制。基质金属蛋白酶家族及其抑制剂是调节这种平衡的机制之一。目前对于调节牙周膜细胞合成胶原酶及其抑制剂TIMP-1的因素了解甚少。本研究旨在使用逆转录聚合酶链反应（RT-PCR）评估白细胞介素-1β（IL-1β）、血小板衍生生长因子（PDGF）和转化生长因子-β1（TGF-β）对牙周来源成纤维细胞中胶原酶（MMP-1）和TIMP-1 mRNA表达的影响。用IL-1β（10和100 pg/ml）、两种PDGF亚型 -AA和 -BB（4和20 ng/ml）以及TGF-β（1和10 ng/ml）处理早期传代的牙周膜来源成纤维细胞。生长因子处理2至24小时的结果显示，对MMP-1 mRNA的最大影响出现在24小时后。IL-1β使MMP-1 mRNA增加了5至9倍。两种PDGF亚型对MMP-1 mRNA的影响较小（3至5倍），而TGF-β使该信使的表达降低了25%至50%。所有生长因子均未对TIMP-1 mRNA表达产生影响。（摘要截短于250字）
