Epp T A, Wang R, Sole M J, Liew C C
Laboratory for Molecular Cardiology, Toronto Hospital, University of Toronto, Ontario, Canada.
J Mol Evol. 1995 Sep;41(3):284-92. doi: 10.1007/BF00186540.
We have recently determined the complete nucleotide sequences of the cardiac alpha- and beta-myosin heavy chain (MyHC) genes from both human and Syrian hamster. These genomic sequence data were used to study the molecular evolution of the cardiac MyHC genes. Between the alpha- and beta-MyHC genes, multiple gene conversion events were detected by (1) maximum parsimony tree analyses, (2) synonymous substitution analyses, and (3) detection of pairwise identity of intron sequences. Approximately half of the 40 cardiac MyHC exons have undergone concerted evolution through the process of gene conversion with the other half undergoing divergent evolution. Gene conversion occurred more often in exons encoding the alpha-helical myosin rod domain than in the globular head domain, and an apparent directional bias was also observed, with transfer of genetic material occurring more often from beta to alpha.
我们最近确定了人类和叙利亚仓鼠心脏α-和β-肌球蛋白重链（MyHC）基因的完整核苷酸序列。这些基因组序列数据被用于研究心脏MyHC基因的分子进化。通过（1）最大简约树分析、（2）同义替换分析和（3）内含子序列的成对同一性检测，在α-和β-MyHC基因之间检测到多个基因转换事件。40个心脏MyHC外显子中约有一半通过基因转换过程经历了协同进化，另一半则经历了趋异进化。基因转换在编码α-螺旋肌球蛋白杆状结构域的外显子中比在球状头部结构域中更频繁发生，并且还观察到明显的方向偏向，遗传物质从β向α的转移更频繁。
