Studies on carrier protein in contact dermatitis: in vivo sensitization with soluble epidermal proteins as carrier proteins.

Contact hypersensitivity to picrylchloride was induced in guinea pigs by the subcutaneous injection of heteroantigenic components which were isolated by antibody immunoadsorbent from soluble epidermal proteins of picrylchloride painted guinea pigs. The injection of non-heterantinenic components obtained from the picrylchloride treated guinea pig epidermis did not elicit contact sensitization to picrylchloride in guinea pigs. These results corroborate previous findings obtained when utilizing the in vitro migration inhibition assay.