Sakai R, Maruta A, Taguchi J, Tomita N, Kodama F, Harada M, Shimizu A, Nakamura Y, Ida M, Kameda Y
Hematology/Chemotherapy, Kanagawa Cancer Center.
Rinsho Ketsueki. 1995 Aug;36(8):735-41.
We investigated the relevance of cytomegalovirus (CMV) to interstitial pneumonitis (IP) using formalin-fixed and paraffin-embedded lung tissues obtained from 12 autopsy cases who died following bone marrow transplantation (BMT) from May 1985 to March in 1992. The lung tissues were examined by hematoxylin and eosin (H.E.) staining, immunohistochemistry with monoclonal antibodies (DDG9, CCH2) and polymerase chain reaction (PCR) for amplifying human CMV DNA. Eight of 12 cases were clinically diagnosed as IP. The 4 cases showed no signs of IP, and their tissues were used as controls. H.E. staining revealed inclusion bodies in two of the eight and only severe fibrosis in the other six. In 4 of the 6 cases, however, CMV was identified by immunohistochemistry or PCR. We concluded that the relevance of CMV in the genesis of IP is often overlooked by H.E. staining alone. Immunohistochemistry and PCR are useful in examining tissues which are suspected of CMV-IP.
我们使用1985年5月至1992年3月期间12例骨髓移植（BMT）后死亡的尸检病例的福尔马林固定石蜡包埋肺组织，研究了巨细胞病毒（CMV）与间质性肺炎（IP）的相关性。通过苏木精和伊红（H.E.）染色、单克隆抗体（DDG9、CCH2）免疫组织化学以及用于扩增人CMV DNA的聚合酶链反应（PCR）对肺组织进行检查。12例中有8例临床诊断为IP。4例未显示IP迹象，其组织用作对照。H.E.染色显示8例中有2例存在包涵体，另外6例仅有严重纤维化。然而，在6例中的4例中，通过免疫组织化学或PCR鉴定出CMV。我们得出结论，单独的H.E.染色常常会忽略CMV在IP发生中的相关性。免疫组织化学和PCR有助于检查疑似CMV-IP的组织。
