Schmidt U, Metz K A, Soukou C, Quabeck K
Department of Pathology, University of Essen, Germany.
Zentralbl Pathol. 1993 Aug;139(3):225-30.
Lung tissue of 104 necropsies was studied by routine (HE) histology, by immunohistochemistry and partly by in situ hybridization in order to explore the association of pulmonary cytomegalovirus (CMV) infection and interstitial pneumonia (IP) after allogeneic bone marrow transplantation. IP was detected in 59 of 104 patients (56%). 12 of these (20% of the IP cases) presented as CMV-IP. No evidence of a CMV infection was obtained in the remaining 47 IP. Immunohistochemistry did not improve the CMV detection essentially over the results of routine (HE) analysis of viral inclusions. In situ hybridization performed on frozen sections of 21 cases turned out to be more sensitive than routine histology and immunohistochemistry, detecting active as well as latent CMV infections. However, the clinical relevance of latent infections, as disclosed by positive hybridization results in the absence of nuclear inclusions as well as immunohistochemical positivities, seems to be low, because latent infections typically were not found to be associated with IP.
对104例尸检的肺组织进行了常规（苏木精-伊红染色，HE）组织学检查、免疫组织化学检查，并部分进行了原位杂交，以探讨异基因骨髓移植后肺巨细胞病毒（CMV）感染与间质性肺炎（IP）之间的关联。104例患者中有59例（56%）检测到IP。其中12例（占IP病例的20%）表现为CMV-IP。其余47例IP未获得CMV感染的证据。与病毒包涵体的常规（HE）分析结果相比，免疫组织化学在CMV检测方面并没有实质性的改善。对21例病例的冰冻切片进行原位杂交，结果显示其比常规组织学和免疫组织化学更敏感，能够检测到活动性和潜伏性CMV感染。然而，在没有核内包涵体以及免疫组织化学阳性的情况下，阳性杂交结果所揭示的潜伏感染的临床相关性似乎较低，因为通常未发现潜伏感染与IP相关。
