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一种用于监测啮齿动物群体中嗜肺巴斯德杆菌抗体的酶联免疫吸附测定(ELISA)。

An enzyme-linked immunosorbent assay (ELISA) for monitoring rodent colonies for Pasteurella pneumotropica antibodies.

作者信息

Boot R, Thuis H C, Veenema J L, Bakker R G

机构信息

Section of Laboratory Animal Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.

出版信息

Lab Anim. 1995 Jul;29(3):307-13. doi: 10.1258/002367795781088306.

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Pasteurella pneumotropica antibodies in the sera of rats, mice, hamsters and Mastomys. P. pneumotropica from mice and rats showed cross-reactivity. The ELISA using P. pneumotropica NCTC 8284 detected more infected animals than selective culture in groups of rodents from which P. pneumotropica, Haemophilus sp and/or Actinobacillus sp were cultured. Cross reactivity between P. pneumotropica NCTC 8284 and haemophilus and actinobacillus isolates were not studied.

摘要

开发了一种酶联免疫吸附测定法(ELISA),用于检测大鼠、小鼠、仓鼠和多乳小鼠血清中的嗜肺巴斯德杆菌抗体。来自小鼠和大鼠的嗜肺巴斯德杆菌显示出交叉反应性。在培养出嗜肺巴斯德杆菌、嗜血杆菌属和/或放线杆菌属的啮齿动物组中,使用嗜肺巴斯德杆菌NCTC 8284的ELISA检测到的感染动物比选择性培养法更多。未研究嗜肺巴斯德杆菌NCTC 8284与嗜血杆菌和放线杆菌分离株之间的交叉反应性。

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