Boot R, Thuis H C, Veenema J L, Bakker R G
Section of Laboratory Animal Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Lab Anim. 1995 Jul;29(3):307-13. doi: 10.1258/002367795781088306.
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Pasteurella pneumotropica antibodies in the sera of rats, mice, hamsters and Mastomys. P. pneumotropica from mice and rats showed cross-reactivity. The ELISA using P. pneumotropica NCTC 8284 detected more infected animals than selective culture in groups of rodents from which P. pneumotropica, Haemophilus sp and/or Actinobacillus sp were cultured. Cross reactivity between P. pneumotropica NCTC 8284 and haemophilus and actinobacillus isolates were not studied.
开发了一种酶联免疫吸附测定法(ELISA),用于检测大鼠、小鼠、仓鼠和多乳小鼠血清中的嗜肺巴斯德杆菌抗体。来自小鼠和大鼠的嗜肺巴斯德杆菌显示出交叉反应性。在培养出嗜肺巴斯德杆菌、嗜血杆菌属和/或放线杆菌属的啮齿动物组中,使用嗜肺巴斯德杆菌NCTC 8284的ELISA检测到的感染动物比选择性培养法更多。未研究嗜肺巴斯德杆菌NCTC 8284与嗜血杆菌和放线杆菌分离株之间的交叉反应性。