Wang G J, Volkow N D, Fowler J S, Ding Y S, Logan J, Gatley S J, MacGregor R R, Wolf A P
Medical Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
Life Sci. 1995;57(14):PL187-91. doi: 10.1016/0024-3205(95)02099-5.
We compared the sensitivity of two dopamine transporter (DAT) ligands ([C-11]cocaine and [C-11]d-threo-methylphenidate) for measurement of extrastriatal DAT availability using positron emission tomography (PET) on separated groups of 10 age matched male volunteers (age range, 21-49 years). DAT availability was obtained using the ratio of the distribution volume in the region of interest to that in the cerebellum (Bmax'/Kd'+ 1). DAT availability measured with [C-11]d-threo-methylphenidate was highest in basal ganglia, followed by thalamus > temporal insula, cingulate > orbitofrontal, frontal and occipital cortices. A similar ranking order for DAT availability was obtained with [C-11]cocaine. Specific binding (Bmax'/Kd') of [C-11]cocaine in thalamus was 25-33% that of basal ganglia and [C-11]d-threo-methylphenidate in thalamus was 11-13% that of basal ganglia. The regional measures with [C-11]cocaine were significantly correlated with those of [C-11]d-threo-methylphenidate (p < or = 0.0001). These results document extrastriatal binding in human brain with two different DAT ligands.
我们使用正电子发射断层扫描（PET），在10名年龄匹配的男性志愿者（年龄范围21 - 49岁）的不同分组中，比较了两种多巴胺转运体（DAT）配体（[C - 11]可卡因和[C - 11]d - 苏式甲基苯丙胺）用于测量纹状体外DAT可用性的敏感性。通过感兴趣区域与小脑的分布体积之比（Bmax'/Kd'+ 1）来获得DAT可用性。用[C - 11]d - 苏式甲基苯丙胺测量的DAT可用性在基底神经节中最高，其次是丘脑>颞岛、扣带回>眶额叶、额叶和枕叶皮质。用[C - 11]可卡因也获得了类似的DAT可用性排名顺序。[C - 11]可卡因在丘脑中的特异性结合（Bmax'/Kd'）是基底神经节的25 - 33%，[C - 11]d - 苏式甲基苯丙胺在丘脑中的特异性结合是基底神经节的11 - 13%。[C - 11]可卡因的区域测量值与[C - 11]d - 苏式甲基苯丙胺的区域测量值显著相关（p≤0.0001）。这些结果证明了两种不同的DAT配体在人脑纹状体外的结合情况。
