Fried B, Nelson P D
Parasitology. 1978 Aug;77(1):49-55. doi: 10.1017/s003118200004871x.
Domestic chicks were infected with Zygocotyle lunata to determine gross and histopathological effects on the caecal tissues, to observe the method of feeding by worms and to examine the effects of crowding on the growth and development of the worm. Caecal weights and dimensions were significantly reduced in infected chicks. Caecal debris, abundant in control chicks, stained positive for proteins, polysaccharides, acid mucopolysaccharides and neutral fats. Caecal debris was rarely seen in infected chicks. The lumen of the intestinal caeca of the worms contained acellular material which showed protease activity and stained positive for proteins, polysaccharides and acid mucopolysaccharides. Histopathological effects of the infection showed a disruption of the architecture of host caecal mucosa, worm to worm attachment, tissue plugs in the worm acetabulum, and an engorgement of host mucosal tissue with erythrocytes. Stunting due to worm crowding was very evident and by 2 weeks post-infection, worms from single-worm infections were sexually mature and more than twice the length of immature flukes obtained from chicks infected with 100-500 cysts.
用月牙形合口吸虫感染雏鸡，以确定对盲肠组织的大体和组织病理学影响，观察蠕虫的摄食方式，并研究拥挤对蠕虫生长发育的影响。感染雏鸡的盲肠重量和尺寸显著减小。对照雏鸡盲肠中有丰富的残渣，对蛋白质、多糖、酸性粘多糖和中性脂肪染色呈阳性。感染雏鸡中很少见到盲肠残渣。蠕虫肠盲管腔内含无细胞物质，该物质具有蛋白酶活性，对蛋白质、多糖和酸性粘多糖染色呈阳性。感染的组织病理学影响表现为宿主盲肠黏膜结构破坏、蠕虫与蠕虫附着、蠕虫吸盘处有组织栓，以及宿主黏膜组织充血并有红细胞。蠕虫拥挤导致的发育迟缓非常明显，感染后2周，单虫感染的蠕虫已性成熟，长度是感染100 - 500个囊蚴的雏鸡体内未成熟吸虫的两倍多。
