Samaha R R, Green R, Noller H F
Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA.
Nature. 1995 Sep 28;377(6547):309-14. doi: 10.1038/377309a0.
Interaction of the conserved CCA terminus of tRNA with rRNA in the peptidyl transferase P site has been studied by in vitro genetics. A watson-Crick G-C pair between G2252 in a conserved hairpin loop of 23S rRNA and C74 at the acceptor end of tRNA is required for proper functional interaction of the CCA end of tRNA with the ribosomal P site. These findings establish a direct role for 23S rRNA in protein synthesis.
通过体外遗传学研究了tRNA保守的CCA末端与肽基转移酶P位点中的rRNA之间的相互作用。23S rRNA保守发夹环中的G2252与tRNA受体末端的C74之间的沃森-克里克G-C对是tRNA的CCA末端与核糖体P位点进行适当功能相互作用所必需的。这些发现确立了23S rRNA在蛋白质合成中的直接作用。
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