Zambardi G, Lecoq M, Reverdy M E, Bes M, Freney J, Fleurette J
Département d'Etudes et de Recherche en Bactériologie (EA 1655), Faculté de Médecine Alexis-Carrel, Lyon, France.
Pathol Biol (Paris). 1995 Apr;43(4):310-4.
The mecA gene which confers the oxacillin resistance has been searched by PCR in 290 (124 positives, 166 negatives) coagulase-negative staphylococci (CNS) belonging to twelve species. The results were compared with the oxacillin MIC values obtained by agar dilution (4% NaCl) or by the ATB STAPH method (Api-bioMérieux; 0%, 2%, 5% NaCl) and growth inhibitory diameters obtained by agar diffusion with an oxacillin disk placed at 30 degrees C without NaCl, or at 35 degrees C in presence of 2% or 5% NaCl. Sensitivity of oxacillin resistance detection depends upon the salt concentration and the method used. The optimum concentration is 2%. With this concentration, the Api ATB test appears as the more performant (sensitivity: 89.8%). Search for the mecA gene by PCR represents a very interesting method that detects 96.9% of the oxacillin-resistant CNS strains.
已通过聚合酶链反应（PCR）在属于12个菌种的290株凝固酶阴性葡萄球菌（CNS，其中124株阳性，166株阴性）中检测了赋予苯唑西林抗性的mecA基因。将结果与通过琼脂稀释法（4%氯化钠）或ATB葡萄球菌检测法（生物梅里埃公司的Api系统；0%、2%、5%氯化钠）获得的苯唑西林最低抑菌浓度（MIC）值，以及在30℃无氯化钠条件下或35℃含2%或5%氯化钠条件下通过苯唑西林纸片琼脂扩散法获得的生长抑制直径进行比较。苯唑西林抗性检测的敏感性取决于盐浓度和所用方法。最佳浓度为2%。在此浓度下，Api ATB试验表现出更高的性能（敏感性：89.8%）。通过PCR检测mecA基因是一种非常有趣的方法，可检测出96.9%的耐苯唑西林CNS菌株。
