Rodriguez P, Fourmaux S, de Barbeyrac B, Rogues A M, Parneix P, Leger A, Bebear C
Laboratoire de Bactériologie, Hôpital Pellegrin, Place Amélie Raba Léon, Bordeaux, France.
Pathol Biol (Paris). 1995 Apr;43(4):352-7.
Because of the emergence of Pseudomonas (Burkholderia) cepacia, isolated among patients from an intensive care unit of the Hôpital Pellegrin (Bordeaux), strains were studied in the aim to determine the source and the mode of transmission of the bacteria. The study was performed on 44 isolates of P. cepacia from 21 patients (23 strains) or from the environment (16 isolates from ventilators and five from the distilled water used for these ventilators). Susceptibility to 23 antibiotics of these strains and endonuclease restriction polymorphism on their total DNA were determined. Pulsed field gel electrophoresis was performed after digestion of the genomic DNA, using three restriction enzymes. Results obtained by analysis of genotypic characteristics demonstrated the clonal origin of the P. cepacia epidemy in the different intensive care units, but did not localize the source of the infection.
由于在波尔多佩勒格林医院重症监护病房的患者中分离出洋葱伯克霍尔德菌（以前称洋葱假单胞菌），因此对菌株进行了研究，以确定细菌的来源和传播方式。该研究对来自21名患者（23株菌株）或环境（来自呼吸机的16株分离株和用于这些呼吸机的蒸馏水中的5株）的44株洋葱伯克霍尔德菌分离株进行。测定了这些菌株对23种抗生素的敏感性及其总DNA的内切酶限制性多态性。使用三种限制性内切酶消化基因组DNA后进行脉冲场凝胶电泳。通过分析基因型特征获得的结果表明，不同重症监护病房中洋葱伯克霍尔德菌流行的克隆起源，但未确定感染源。
