Yan He, Shi Lei, Alam M J, Li Lin, Yang Liansheng, Yamasaki Shinji
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
Microbiol Immunol. 2008 May;52(5):283-6. doi: 10.1111/j.1348-0421.2008.00035.x.
The aim of the present study was to determine the source of nosocomial outbreak due to Burkholderia cepacia by molecular techniques. A total of 11 B. cepacia strains were isolated; nine from blood and one from sputum of patients without cystic fibrosis, and one from reverse osmosis water at a local hospital in Guangzhou, China. Analyses of 11 strains by the Sau-PCR assay and pulsed-field gel electrophoresis revealed that nine strains obtained from the blood of outpatients in a hemodialysis unit and one strain from reverse osmosis water had identical DNA profiles, indicating that the reverse osmosis water supply could be a source of infection.
本研究的目的是通过分子技术确定洋葱伯克霍尔德菌引起医院感染暴发的来源。共分离出11株洋葱伯克霍尔德菌；9株来自无囊性纤维化患者的血液，1株来自痰液，1株来自中国广州一家当地医院的反渗透水。通过Sau-PCR分析和脉冲场凝胶电泳对11株菌株进行分析，结果显示，从血液透析单元门诊患者血液中分离出的9株菌株和从反渗透水中分离出的1株菌株具有相同的DNA图谱，表明反渗透水供应可能是感染源。
