Hamaguchi A, Tooyama I, Yoshiki T, Kimura H
Department of Urology, Shiga University of Medical Science, Otsu, Japan.
Prostate. 1995 Sep;27(3):141-7. doi: 10.1002/pros.2990270304.
The expression and localization of fibroblast growth factor receptor-1 were investigated in human prostatic tissues with or without benign hyperplasia. Using a polymerase chain reaction method, we were able to demonstrate that prostatic tissues with benign hyperplasia expressed a significantly higher level of fibroblast growth factor receptor-1 mRNA than normal prostatic tissues (P < 0.01 by Anova). Western blot analysis using an antiserum against the receptor gave 2 bands with molecular weights of about 140 kDa and 80 kDa; these correspond to the expected sizes of the long and secreted forms of the fibroblast growth factor receptor-1, respectively. An immunohistochemical study using the same antiserum further demonstrated that the immunoreactive staining occurred mainly in the basal cells of the glandular epithelium and occasionally in the stromal cells. These results suggest that fibroblast growth factors may influence, at least in part, the proliferation of the epithelial cells seen in benign hyperplasia of human prostate.
在有或无良性增生的人前列腺组织中研究了成纤维细胞生长因子受体-1的表达和定位。使用聚合酶链反应方法，我们能够证明有良性增生的前列腺组织比正常前列腺组织表达显著更高水平的成纤维细胞生长因子受体-1 mRNA（方差分析，P<0.01）。使用针对该受体的抗血清进行的蛋白质免疫印迹分析产生了两条带，分子量分别约为140 kDa和80 kDa；这些分别对应于成纤维细胞生长因子受体-1的长形式和分泌形式的预期大小。使用相同抗血清的免疫组织化学研究进一步证明，免疫反应性染色主要发生在腺上皮的基底细胞中，偶尔也发生在基质细胞中。这些结果表明，成纤维细胞生长因子可能至少部分地影响人前列腺良性增生中所见上皮细胞的增殖。
