Ghosh P K, Castellanos-Barba C, Ortiz-Ortiz L
Departamento de Inmunología, Universidad Nacional Autónoma de México, México, D. F.
Parasitol Res. 1995;81(6):475-80. doi: 10.1007/BF00931789.
The cellular immune response was evaluated in a C3H/HeJ mouse model of intestinal amebiasis at 5-60 days postinoculation with Entamoeba histolytica. At various intervals, spleen lymphocytes were obtained from infected and noninfected control mice and cultured with concanavalin A (Con A), pokeweed mitogen (PWM), or ameba antigen. E. histolytica infection induced a cyclic depression of DNA synthesis when spleen lymphocytes were stimulated with a T-cell mitogen (Con A), a T- and B-cell (PWM) mitogen, or an ameba antigen. A similar response was observed in the determinations of interleukin-2 in the supernatants of Con A-stimulated spleen cells from infected mice. When spleen cells from E. histolytica-infected mice were stimulated with phorbol myristate acetate plus ionomycin, results indicated a signal-transduction defect. These alterations, observed at the cellular level, might facilitate invasion of the host by the parasite.
在用溶组织内阿米巴接种后5至60天，在C3H/HeJ小鼠肠道阿米巴病模型中评估细胞免疫反应。在不同时间间隔，从感染和未感染的对照小鼠中获取脾淋巴细胞，并用刀豆球蛋白A（Con A）、商陆有丝分裂原（PWM）或阿米巴抗原进行培养。当脾淋巴细胞用T细胞有丝分裂原（Con A）、T和B细胞（PWM）有丝分裂原或阿米巴抗原刺激时，溶组织内阿米巴感染诱导DNA合成的周期性抑制。在对感染小鼠Con A刺激的脾细胞上清液中的白细胞介素-2的测定中也观察到类似反应。当用佛波酯肉豆蔻酸酯加离子霉素刺激溶组织内阿米巴感染小鼠的脾细胞时，结果表明存在信号转导缺陷。在细胞水平观察到的这些改变可能有助于寄生虫对宿主的侵袭。
