Field J S, Irwin S W
School of Applied Biological and Chemical Sciences, University of Ulster at Jordanstown, Antrim, Northern Ireland, UK.
Parasitol Res. 1995;81(6):505-17. doi: 10.1007/BF00931794.
Precise identification of members of the genus Diplostomum is difficult in that many larval stages have been described and named separately from their adult forms. In this study, the life cycles of two Diplostomum spp. were established using metacercariae obtained from the eyes of rainbow trout. The parasites were identified as D. spathaceum and D. pseudobaeri using a procedure and key devised by Shigin. Each life cycle was completed in the laboratory using domestic chickens (Gallus gallus domesticus L.) as definitive hosts and Lymnaea peregra (Muller) and rainbow trout as first and second intermediate hosts, respectively. Utilizing identical hosts minimised host-induced morphological variations and allowed comparisons of anatomical features to be made. Light and scanning electron microscopy were used to study each developmental stage and detailed descriptions of the life cycles were compiled. The results obtained may go some way to resolving the confusion that surrounds Diplostomum taxonomy.
双穴吸虫属成员的精确鉴定很困难，因为许多幼虫阶段是与其成虫形态分开描述和命名的。在本研究中，利用从虹鳟鱼眼睛中获得的尾蚴，确定了两种双穴吸虫的生活史。使用Shigin设计的程序和检索表，将这些寄生虫鉴定为匙形双穴吸虫和伪贝氏双穴吸虫。每个生活史在实验室中完成，分别以家鸡（Gallus gallus domesticus L.）作为终末宿主，以静水椎实螺（Lymnaea peregra (Muller)）和虹鳟鱼作为第一和第二中间宿主。利用相同的宿主可将宿主诱导的形态变异降至最低，并便于对解剖特征进行比较。使用光学显微镜和扫描电子显微镜研究每个发育阶段，并编制了生活史的详细描述。所获得的结果可能在一定程度上解决围绕双穴吸虫分类学的混乱问题。
