Gala R R
Department of Physiology, Wayne State University, School of Medicine, Detroit, Michigan 48201, USA.
Proc Soc Exp Biol Med. 1995 Nov;210(2):117-25. doi: 10.3181/00379727-210-43930.
A number of immune parameters were examined in Snell dwarf mice and compared with normal littermates. The number of splenocytes per gram of body weight were significantly decreased in dwarf animals, and the decrease was distributed throughout the CD4, CD8, B220, and MAC-1 subsets. The percentage of CD4 and CD8 splenocytes was markedly increased, and the percentage of B220 and MAC-1 splenocytes markedly decreased, in dwarf animals. In addition, the percentage of splenocyte T cells constitutively expressing interleukin-2 (IL-2) receptors and prolactin (PRL) receptors was decreased, with the CD4 subset presenting the most dramatic effect. The effects of replacing the hormones deficient in the Snell dwarf mouse (i.e., growth hormone [GH], prolactin [PRL], and thyroxine [T4] on the above immune parameters were also examined. The administration of T4 alone for 10 days corrected the defect in splenocyte cell numbers per grams body weight for both the CD4 and CD8 subsets, but only partially corrected the defect for the B220 and MAC-1 subsets. The addition of rbGH and rbPRL for the last 3 days of T4 injection had little additive effect on the number of CD4 and CD8 cells but increased the number of B220 and MAC-1 subsets to values comparable to those of normal animals on the basis of body weight. The decrease in the percentage of CD4 splenocytes in dwarf animals constitutively expressing IL-2R was partially corrected by T4 injection and completely corrected by the addition of rbGH and rbPRL for the last 3 days. The decrease in CD4 splenocytes constitutively expressing PRLR was partially corrected by T4 injection alone and the addition of rbGH and rPRL resulted in percentages comparable to that of normal animals. The results indicate that Snell dwarf animals are deficient in immune parameters and that the administration of the hormones lacking in this animal can correct the deficiencies.
对斯奈尔侏儒小鼠的多项免疫参数进行了检测，并与正常同窝小鼠进行了比较。侏儒动物每克体重的脾细胞数量显著减少，且这种减少分布于整个CD4、CD8、B220和MAC-1亚群。侏儒动物中，CD4和CD8脾细胞的百分比显著增加，而B220和MAC-1脾细胞的百分比显著降低。此外，组成性表达白细胞介素-2（IL-2）受体和催乳素（PRL）受体的脾细胞T细胞百分比降低，其中CD4亚群受影响最为显著。还研究了补充斯奈尔侏儒小鼠所缺乏的激素（即生长激素[GH]、催乳素[PRL]和甲状腺素[T4]）对上述免疫参数的影响。单独给予T4 10天可纠正CD4和CD8亚群每克体重脾细胞数量的缺陷，但仅部分纠正B220和MAC-1亚群的缺陷。在注射T4的最后3天添加重组人生长激素（rbGH）和重组人催乳素（rbPRL）对CD4和CD8细胞数量几乎没有附加作用，但根据体重，可使B220和MAC-1亚群的数量增加至与正常动物相当的值。注射T4可部分纠正侏儒动物中组成性表达IL-2R的CD4脾细胞百分比的降低，在最后3天添加rbGH和rbPRL可完全纠正。单独注射T4可部分纠正组成性表达PRLR的CD4脾细胞的减少，添加rbGH和rPRL可使百分比与正常动物相当。结果表明，斯奈尔侏儒动物存在免疫参数缺陷，给予该动物所缺乏的激素可纠正这些缺陷。
