Gala R R, Shevach E M
Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201.
Proc Soc Exp Biol Med. 1993 Nov;204(2):224-30. doi: 10.3181/00379727-204-43657.
The influence of recombinant bovine prolactin (PRL) and recombinant bovine growth hormone (GH) was examined on the popliteal lymph node (PLN) expression of interleukin-2 receptors (IL-2R) in female Snell dwarf mice and normal litter mates after concanavalin A footpad injection. The absolute number of PLN CD4+, CD8+, or B+ cells of dwarf mice was less than that observed for normal litter mates, but when adjusted for the difference in body weight, only the absolute number of B cells was lower in dwarf animals when compared with normal litter mates. The injection of PRL or GH did not alter the observation. The administration of recombinant bovine PRL to normal animals, but not recombinant bovine GH, increased the expression of IL-2R or unstimulated PLN CD4+ and CD8+ subsets. Hormone administration to dwarf animals, however, did not alter the expression of IL-2R on unstimulated PLN T cell subsets. PLN cells from dwarf animals were poorly activated in vivo after injection of concanavalin A and the level of IL-2R expression induced was only 50% of that seen in the PLN of normal animals. The administration of PRL and GH completely corrected the defective induction of IL-2R expression on PLN from dwarf animals after concanavalin A stimulation. These findings strongly suggest that PRL and/or GH play an important role at some stage of the T cell activation process in vivo. Further studies are needed to precisely identify the defect in the dwarf mice.
在雌性斯奈尔侏儒小鼠和正常同窝仔鼠中，通过注射刀豆蛋白A至足垫，研究重组牛催乳素（PRL）和重组牛生长激素（GH）对腘窝淋巴结（PLN）白细胞介素-2受体（IL-2R）表达的影响。侏儒小鼠PLN中CD4 +、CD8 +或B +细胞的绝对数量低于正常同窝仔鼠，但根据体重差异进行调整后，与正常同窝仔鼠相比，侏儒动物中只有B细胞的绝对数量较低。注射PRL或GH并未改变这一观察结果。给正常动物注射重组牛PRL而非重组牛GH，可增加未刺激的PLN中CD4 +和CD8 +亚群的IL-2R表达。然而，给侏儒动物注射激素并未改变未刺激的PLN T细胞亚群上IL-2R的表达。注射刀豆蛋白A后，侏儒动物的PLN细胞在体内的活化较差，诱导的IL-2R表达水平仅为正常动物PLN中所见水平的50%。PRL和GH的注射完全纠正了刀豆蛋白A刺激后侏儒动物PLN上IL-2R表达的缺陷诱导。这些发现强烈表明，PRL和/或GH在体内T细胞活化过程的某个阶段发挥重要作用。需要进一步研究以精确确定侏儒小鼠中的缺陷。
