Hawkins T A, Gala R R, Dunbar J C
Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
J Reprod Immunol. 1996 Nov;32(1):55-71. doi: 10.1016/s0165-0378(96)00986-2.
The NOD mouse is a model for autoimmune diabetes that develops symptoms similar to Type I diabetes. The incidence of diabetes is greater in females but the degree of insulitis is comparable in both sexes. The purpose of this study was to assess the populations of lymphocytes and macrophages in the pancreas and spleen of NOD mice. Comparisons were made between male and female; young (32-40 days old) and old (197-297 days old); diabetic and non-diabetic mice. Using analytical fluorescent cell cytometry we quantitated the percentages of CD4, and CD8 T-cells, B-cells and macrophages and the percentages of these subsets expressing interleukin-2 (IL-2R), prolactin (PRLR) and Hi-intensity PRL (Hi-PRLR) receptors. Evaluation of T-splenocytes indicated a 2:1 ratio of CD4 to CD8 T-lymphocytes in the spleen. The pancreas had higher percentages of all of the subsets in the old male and female groups compared to their young counterparts. Pancreatic immunocompetent cell subsets expressed lower percentages of IL-2R, PRLR and Hi-PRLR compared to splenocytes. The results did not demonstrate any dramatic differences in the immunocompetent cell populations of the spleen or pancreas between male and female animals, however we were able to establish the percentage of immunocompetent cells with IL-2R, PRLR and Hi-PRLR as a reference for future studies.
NOD小鼠是一种自身免疫性糖尿病模型，会出现与I型糖尿病相似的症状。糖尿病在雌性小鼠中的发病率更高，但两性的胰岛炎程度相当。本研究的目的是评估NOD小鼠胰腺和脾脏中淋巴细胞和巨噬细胞的数量。对雄性和雌性、年轻（32 - 40日龄）和年老（197 - 297日龄）、糖尿病和非糖尿病小鼠进行了比较。使用分析型荧光细胞仪，我们定量了CD4、CD8 T细胞、B细胞和巨噬细胞的百分比，以及这些亚群中表达白细胞介素-2（IL-2R）、催乳素（PRLR）和高强度PRL（Hi-PRLR）受体的百分比。对脾T细胞的评估表明，脾脏中CD4与CD8 T淋巴细胞的比例为2:1。与年轻组相比，老年雄性和雌性组胰腺中所有亚群的百分比更高。与脾细胞相比，胰腺免疫活性细胞亚群表达的IL-2R、PRLR和Hi-PRLR百分比更低。结果并未显示雄性和雌性动物脾脏或胰腺的免疫活性细胞数量有任何显著差异，然而我们能够确定表达IL-2R、PRLR和Hi-PRLR的免疫活性细胞百分比，作为未来研究的参考。
