Inoue M, Funaba M, Hawkins R L, Mori M, Torii K
Torii Nutrient-Stasis Project, Research Development Corporation of Japan, Yokohama.
Physiol Behav. 1995 Aug;58(2):379-85. doi: 10.1016/0031-9384(95)00073-r.
The effect of continuous L-lysine (Lys) infusion on dietary choice between Lys deficient and protein-free diets in Sprague-Dawley rats was studied to determine the sensing site of Lys deficiency. After daily intake of each diet became constant, Lys was continuously infused for 11 days via intraperitoneal (IP), intragastric (IG) or intracerebroventricular (ICV) route, with an osmotic pump. Daily intake of each diet was measured. Intake of the Lys deficient diet compared with protein-free diet in either IP or IG Lys-infused group increased significantly (p < 0.001) vs. the intake in the baseline period. The selection of the Lys deficient diet was quite comparable between IP and IG groups. But the intake of the ICV group was unchanged. Hepatic vagotomy during IP infusion transiently delayed selection of the Lys deficient diet. These results imply the roles of postabsorptive mechanisms in sensing an amino acid deficiency, and possible involvement of the hepatic branch of the vagus in the sensing. However, sensing in the brain or indeed in the intestine was not excluded.
研究了连续输注L-赖氨酸（Lys）对斯普拉格-道利大鼠在赖氨酸缺乏饮食和无蛋白饮食之间饮食选择的影响，以确定赖氨酸缺乏的感知部位。在每种饮食的每日摄入量变得恒定后，通过腹腔内（IP）、胃内（IG）或脑室内（ICV）途径，使用渗透泵连续输注Lys 11天。测量每种饮食的每日摄入量。与无蛋白饮食相比，IP或IG输注Lys组中赖氨酸缺乏饮食的摄入量与基线期相比显著增加（p < 0.001）。IP组和IG组之间对赖氨酸缺乏饮食的选择相当。但ICV组的摄入量没有变化。IP输注期间进行肝迷走神经切断术会暂时延迟对赖氨酸缺乏饮食的选择。这些结果表明吸收后机制在感知氨基酸缺乏中的作用，以及迷走神经肝支可能参与感知。然而，并不排除在大脑或肠道中的感知作用。
