Pardo A G, Forchiassin F
Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Rev Argent Microbiol. 1998 Jan-Mar;30(1):20-9.
The production of the extracellular cellulolytic enzyme system (endoglucanase, exoglucanase and cellobiase) of N. catalinensis was tested with different nitrogen sources, inorganic and organic ones, in liquid culture medium with microcrystalline cellulose. The nitrogen compounds used were: potassium nitrate, sodium nitrate, ammonium nitrate, ammonium phosphate, ammonium sulphate, ammonium chloride, ammonium carbonate, ammonium acetate, ammonium tartrate, urea, casamino acids, glycine, L-alanine, L-leucine, L-proline, L-lysine, L-aspartic acid, L-glutamic acid, L-asparagine, L-glutamine, L-phenylalanine, L-tyrosine, L-tryptophan, L-methionine and L-cysteine. Among these, ammonium nitrate and ammonium tartrate gave the highest yields of cellulases in 20-day-old cultures at a concentration equivalent to 0.75 g N/l in both cases. Optimal temperature for cellulase production, growth and cellulose degradation was 23 degrees C. On the other hand, an initial pH of 6.5 gave the highest yields of endoglucanase and cellobiase. In the same way, at pH 6.5, maximal growth and cellulose degradation were achieved. However, maximal exoglucanase production and glycogen content were reached at pH 7.5.
在含有微晶纤维素的液体培养基中，用不同的氮源（无机氮源和有机氮源）测试了卡塔林纳假丝酵母胞外纤维素酶系统（内切葡聚糖酶、外切葡聚糖酶和纤维二糖酶）的产生。所使用的含氮化合物有：硝酸钾、硝酸钠、硝酸铵、磷酸铵、硫酸铵、氯化铵、碳酸铵、醋酸铵、酒石酸铵、尿素、酪蛋白氨基酸、甘氨酸、L-丙氨酸、L-亮氨酸、L-脯氨酸、L-赖氨酸、L-天冬氨酸、L-谷氨酸、L-天冬酰胺、L-谷氨酰胺、L-苯丙氨酸、L-酪氨酸、L-色氨酸、L-蛋氨酸和L-半胱氨酸。其中，硝酸铵和酒石酸铵在20日龄培养物中，在两种情况下浓度均相当于0.75 g N/l时，纤维素酶产量最高。纤维素酶产生、生长和纤维素降解的最适温度为23℃。另一方面，初始pH值为6.5时，内切葡聚糖酶和纤维二糖酶的产量最高。同样，在pH值为6.5时，实现了最大生长和纤维素降解。然而，在pH值为7.5时，外切葡聚糖酶产量和糖原含量达到最高。
