Iannello R C, Young J C, Sumarsono S, Tymms M J, Kola I
Centre for Early Human Development, Monash Medical Centre, Clayton, Vic, Australia.
Reprod Fertil Dev. 1994;6(5):599-604. doi: 10.1071/rd9940599.
Spermatogenesis is a complex process requiring the coordinate expression of a number of testis-specific genes. One of these, Pdha-2, codes for the murine testis-specific isoform of the E1 alpha subunit of the pyruvate dehydrogenase complex. To elucidate the mechanisms regulating its expression in vivo, we have begun to investigate the Pdha-2 promoter in transgenic mice. In this paper, a construct containing 3.0 kb of promoter and upstream sequences is reported to be sufficient for directing the testis-specific expression of a CAT reporter gene in mice harbouring the transgene. Similarly to the endogenous Pdha-2, the CAT gene is expressed in testis in a stage-specific manner. However, the 3.0-kb Pdha-2 promoter is not active in somatic tissue suggesting that repressor elements may be present within these sequences.
精子发生是一个复杂的过程，需要许多睾丸特异性基因的协调表达。其中之一，Pdha-2，编码丙酮酸脱氢酶复合体E1α亚基的小鼠睾丸特异性同工型。为了阐明体内调节其表达的机制，我们已开始在转基因小鼠中研究Pdha-2启动子。本文报道，含有3.0 kb启动子和上游序列的构建体足以指导携带转基因的小鼠中CAT报告基因的睾丸特异性表达。与内源性Pdha-2相似，CAT基因在睾丸中以阶段特异性方式表达。然而，3.0 kb的Pdha-2启动子在体细胞组织中无活性，这表明这些序列中可能存在抑制元件。
