Pierart J, Oñate E, Klaassen R, Cid L, Gutierrez S, Talbot E, Ross E, Zambrano C, Burmeister R, Puchi M
Departamentos de Cirugía y Anatomía Patológica, Facultad de Medicina, Universidad de Concepción, Chile.
Rev Med Chil. 1995 Feb;123(2):165-75.
Tyrosine protein kinase (TPK) activity is associated to malignant cellular transformation. This work compares TPK activity in 27 surgical biopsy samples of mammary carcinoma, 10 samples of fibroadenomas, 13 samples of fibrocystic breast disease and 27 samples of normal mammary tissue. TPK activity was determined in tissue homogenates using (Val5) angiotensin II as exogenous substrate. In samples of mammary carcinoma, TPK activity was 33.86 +/- 31.98 pmol P32/mg protein/30 min. This value was significantly higher that those observed in fibrocystic disease (3.92 +/- 2.35), fibroadenomas (13.86 +/- 10.9) and normal tissue (3.56 +/- 3.02).
酪氨酸蛋白激酶（TPK）活性与恶性细胞转化有关。这项研究比较了27份乳腺癌手术活检样本、10份纤维腺瘤样本、13份乳腺纤维囊性疾病样本和27份正常乳腺组织样本中的TPK活性。使用（缬氨酸5）血管紧张素II作为外源性底物，在组织匀浆中测定TPK活性。在乳腺癌样本中，TPK活性为33.86±31.98皮摩尔P32/毫克蛋白质/30分钟。该值显著高于在纤维囊性疾病（3.92±2.35）、纤维腺瘤（13.86±10.9）和正常组织（3.56±3.02）中观察到的值。
