Dahlberg K R, Cotter D A
Microbios. 1978;23(93-94):153-66.
Freshly formed wild type Dictyostelium discoideum spores are constitutively dormant, and thus require an activation treatment to germinate. Wild type spores may germinate without an activation treatment (autoactivate) after a period of ageing (maturation) in the intact fruiting body. Mutants have been isolated which autoactivate without the need for ageing. Autoactivation of mutant and aged wild type spores appears to occur by identical mechanisms; thus the mutation may involve premature maturation. Autoactivation is mediated by autoactivator substances released from spores as they spontaneously swell. These factors are readily chromatographed, and elute from a Biogel P2 column in three peaks of activity. One activity peak appears only after spores have begun to germinate. No autoactivator substances are released from heat activated spores. Autoactivation is sensitive to cychloheximide, and may result from altered spore permeability. Autoactivation is likely to be the mechanism of D. discoideum spore germination in nature.
新形成的野生型盘基网柄菌孢子处于组成型休眠状态，因此需要进行激活处理才能萌发。野生型孢子在完整子实体中经过一段时间的老化（成熟）后，可能无需激活处理即可萌发（自动激活）。已经分离出了无需老化就能自动激活的突变体。突变体和老化野生型孢子的自动激活似乎通过相同的机制发生；因此，该突变可能涉及过早成熟。自动激活是由孢子在自发膨胀时释放的自动激活物质介导的。这些因子很容易通过色谱分离，并从Biogel P2柱上以三个活性峰的形式洗脱下来。一个活性峰仅在孢子开始萌发后出现。热激活的孢子不会释放自动激活物质。自动激活对放线菌酮敏感，可能是孢子通透性改变所致。自动激活可能是盘基网柄菌孢子在自然条件下萌发的机制。
