Sato Y, Kobayashi J, Shiroma Y
Department of Parasitology, School of Medicine, University of the Ryukyus, Okinawa, Japan.
Rev Inst Med Trop Sao Paulo. 1995 Jan-Feb;37(1):35-41. doi: 10.1590/s0036-46651995000100006.
Parasitological diagnosis based on the faecal examination is frequently difficult in cases of chronic, low-level S. stercoralis infection. Even when a newly developed sensitive method (an agar plate culture) is applied, it is essential to examine faecal samples repeatedly to achieve a correct diagnosis. Additionally, it is important to note that a negative result does not necessarily indicate the unequivocal absence of the infection. On the other hand, several serological tests which have recently been developed for strongyloidiasis have proven reliable when used to complement parasitological examination. We have developed two serological tests, ELISA and GPAT, to demonstrate Strongyloides infection and possible applications of the serological tests for diagnosis, mass-screening, epidemiological study and postchemotherapy evaluation of strongyloidiasis were reviewed based on our recent studies.
对于慢性、低水平粪类圆线虫感染病例，基于粪便检查的寄生虫学诊断常常困难。即便应用新开发的敏感方法（琼脂平板培养法），反复检查粪便样本对于做出正确诊断也至关重要。此外，需注意阴性结果不一定表明明确没有感染。另一方面，最近开发的几种用于类圆线虫病的血清学检测，在用于补充寄生虫学检查时已证明可靠。我们开发了两种血清学检测方法，即酶联免疫吸附测定（ELISA）和糖蛋白抗原检测（GPAT），以证实类圆线虫感染，并基于我们最近的研究对血清学检测在类圆线虫病诊断、大规模筛查、流行病学研究及化疗后评估中的可能应用进行了综述。
