Sato Y, Kobayashi J, Toma H, Shiroma Y
Department of Parasitology, School of Medicine, University of the Ryukyus, Okinawa, Japan.
Am J Trop Med Hyg. 1995 Sep;53(3):248-50. doi: 10.4269/ajtmh.1995.53.248.
To determine the efficacy of stool examination for detection of Strongyloides infection, 1,350 stool samples collected in Japan, Brazil, and Thailand were examined by four different methods (direct fecal smear, formalin-ether concentration. Harada-Mori filter paper culture, and agar plate culture). The newly developed agar plate culture method was highly effective; more than 96% of the positive cases were diagnosed by this method. The coprologic examination, however, was not sensitive enough for detecting chronic infections because more than 40% of the positive cases were overlooked even when persons with proven Strongyloides infection were re-examined several months later without intervening treatment. Therefore, it is essential to examine stool samples repeatedly to achieve a correct diagnosis, and even so, it is important to note that a negative result does not necessarily indicate the unequivocal absence of the infection.
为确定粪便检查对检测类圆线虫感染的效果，对在日本、巴西和泰国采集的1350份粪便样本采用四种不同方法（直接粪便涂片法、福尔马林-乙醚浓缩法、原田-森氏滤纸培养法和琼脂平板培养法）进行检测。新开发的琼脂平板培养法效果极佳；超过96%的阳性病例通过该方法得以诊断。然而，粪便学检查对于检测慢性感染不够敏感，因为即使对已确诊为类圆线虫感染的患者在数月后未经干预治疗的情况下进行复查，仍有超过40%的阳性病例被漏检。因此，反复检查粪便样本对于做出正确诊断至关重要，即便如此，仍需注意阴性结果并不一定表明绝对没有感染。
