巴西副球孢子菌。对从犰狳（九带犰狳）分离出的一份样本进行的真菌学和免疫化学研究。

Paracoccidioides brasiliensis. A mycologic and immunochemical study of a sample isolated from an armadillo (Dasipus novencinctus).

作者信息

Vidal M S, de Melo N T, Garcia N M, Del Negro G M, de Assis C M, Heins-Vaccari E M, Naiff R D, Mendes R P, Lacaz C da S

机构信息

Laboratório de Micologia Médica, Instituto de Medicina Tropical de São Paulo, Brasil.

出版信息

Rev Inst Med Trop Sao Paulo. 1995 Jan-Feb;37(1):43-9. doi: 10.1590/s0036-46651995000100007.

DOI:10.1590/s0036-46651995000100007

PMID:7569639

Abstract

A sample of P. brasiliensis isolated from the spleen and the liver of an armadillo (Dasipus novencinctus) has been analysed under a mycological and immunochemical viewpoint. The armadillo was captured in an area of Tucuruí (State of Pará, Brazil), the animal being already established as an enzootic reservoir of P. brasiliensis at that region of the country. This sample maintained in the fungal collection of the Tropical Medicine Institute of São Paulo (Brazil) numbered 135, has got all the characteristics of P. brasiliensis, with a strong antigenic power and low virulence for guinea-pigs and Wistar rats. The specific exoantigen of P. brasiliensis--the glycoprotein with a molecular weight of 43 kDa--was easily demonstrated with double immunodiffusion, immunoelectrophoresis, SDS-PAGE and immunobloting techniques.

摘要

从一只犰狳（九带犰狳）的脾脏和肝脏中分离出的巴西副球孢子菌样本，已从真菌学和免疫化学角度进行了分析。这只犰狳在图库里伊地区（巴西帕拉州）捕获，在该国该地区，这种动物已被确认为巴西副球孢子菌的动物疫源地。保存在巴西圣保罗热带医学研究所真菌收藏中的该样本编号为135，具有巴西副球孢子菌的所有特征，对豚鼠和Wistar大鼠具有很强的抗原性和低毒力。巴西副球孢子菌的特异性外抗原——分子量为43 kDa的糖蛋白——通过双向免疫扩散、免疫电泳、SDS - PAGE和免疫印迹技术很容易得到证实。