精液中细胞成分的中性红超活染色法
Neutral red supravital staining for cellular elements in the semen.
作者信息
Phadke A M
出版信息
Andrologia. 1978 Jan-Feb;10(1):80-4. doi: 10.1111/j.1439-0272.1978.tb01322.x.
Human seminal fluid besides spermatozoa often contains other cellular elements. A supravital staining method designed to differentiate the above mentioned cellular elements was described. Amongst the spermatogenic cells only spermatocytes were stained with Neutral Red. They displayed two peculiar structures designated as "Y" granules and "Enigmatic Body". Neutral Red was absorbed by the spermiophage cells and was concentrated by them in the form of cytoplasmic granules. In addition the coarse granules of leukocytes and the gigestive vacuoles of Balantidium Coli and Trichomonads were stained with Neutral Red.
人类精液除精子外通常还含有其他细胞成分。本文描述了一种用于区分上述细胞成分的超活染色方法。在生精细胞中，只有精母细胞被中性红染色。它们呈现出两种特殊结构，分别称为“Y”颗粒和“神秘小体”。中性红被噬精细胞吸收，并以细胞质颗粒的形式在细胞内浓缩。此外，白细胞的粗大颗粒以及结肠小袋纤毛虫和滴虫的消化泡也被中性红染色。
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