Leubitz S S, Savage R A
Am J Clin Pathol. 1984 Jan;81(1):90-3. doi: 10.1093/ajcp/81.1.90.
A serial dilution study from human ejaculate was done in order to assess the sensitivity of a commonly employed stain, picroindigocarmine/nuclear fast red, for the detection of spermatozoa. The results of the study indicate that spermatozoa may be detected readily at very low concentrations by scanning 20 high-power fields of a stained smear.
为了评估一种常用染色剂——苦味靛胭脂/核固红——检测精子的敏感性,对人类精液进行了系列稀释研究。研究结果表明,通过扫描染色涂片的20个高倍视野,在非常低的浓度下也能很容易地检测到精子。