Bacon K B, Premack B A, Gardner P, Schall T J
Department of Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA.
Science. 1995 Sep 22;269(5231):1727-30. doi: 10.1126/science.7569902.
The chemokine RANTES induced biphasic mobilization of Ca2+ in T cells. The initial peak, a transient increase in cytosolic Ca2+ mediated by a heterotrimeric guanine nucleotide-binding protein (G protein)--coupled pathway, was associated predominantly with chemotaxis. The second peak, Ca2+ release and sustained influx dependent on protein tyrosine kinases, was associated with a spectrum of cellular responses--Ca2+ channel opening, interleukin-2 receptor expression, cytokine release, and T cell proliferation--characteristic of T cell receptor activation. Other chemokines did not produce these responses. Thus, in addition to inducing chemotaxis, RANTES can act as an antigen-independent activator of T cells in vitro.
趋化因子RANTES可诱导T细胞内Ca2+发生双相性动员。最初的峰值，即由异三聚体鸟嘌呤核苷酸结合蛋白（G蛋白）偶联途径介导的胞质Ca2+的短暂升高，主要与趋化作用相关。第二个峰值，即依赖蛋白酪氨酸激酶的Ca2+释放和持续内流，与一系列细胞反应相关——Ca2+通道开放、白细胞介素-2受体表达、细胞因子释放以及T细胞增殖——这些都是T细胞受体激活的特征。其他趋化因子不会产生这些反应。因此，除了诱导趋化作用外，RANTES在体外还可作为T细胞的抗原非依赖性激活剂。
