Hwang J, Tamm L K, Ramalingam T S, Betzig E, Edidin M
Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Science. 1995 Oct 27;270(5236):610-4. doi: 10.1126/science.270.5236.610.
Near-field scanning optical microscopy of phospholipid monolayers doped with fluorescent lipid analogs reveals previously undescribed features in various phases, including a concentration gradient at the liquid-expanded/liquid-condensed domain boundary and weblike structures in the solid-condensed phase. Presumably, the web structures are grain boundaries between crystalline solid lipid. These structures are strongly modulated by the addition of low concentrations of cholesterol and ganglioside GM1 in the monolayer.
对掺杂荧光脂质类似物的磷脂单层进行近场扫描光学显微镜观察，揭示了不同相中以前未描述的特征，包括在液体扩张/液体凝聚域边界处的浓度梯度以及固体凝聚相中的网状结构。据推测，网状结构是结晶固体脂质之间的晶界。这些结构在单层中通过添加低浓度的胆固醇和神经节苷脂GM1受到强烈调节。
