Li M Y, Wang Z L, Lu G Q
Department of Physiology, Sun Yat-Sen University of Medical Sciences, Guangzhou.
Sheng Li Xue Bao. 1995 Jun;47(3):259-63.
The present experiment is undertaken to study the central mechanism of the inhibitory effect of 2-pyridylethylamine (PEA, i.c.v.) on the gastric acid secretion. Gastric acid was continuously washed out with 37 degrees C saline by means of a perfusion pump in male adrenalectomized wistar rats. Drugs were injected into the third ventricle to examine the effect on pentagastrin-induced (160 micrograms/kg, s.c.) gastric acid secretion. The results were as follows: (1) Pretreatment with CRF-antiserum (2.5 microliters, 1:20,000, i.c.v.) blocked the inhibitory effect of PEA (10 micrograms, i.c.v.) on gastric acid secretion. (2) Administration of CRF (1 and 2 micrograms, i.c.v.) or beta-endorphin (0.94 and 1.25 micrograms, i.c.v.) markedly inhibited the gastric acid secretion. (3) Pretreatment with naloxone (5 micrograms, i.c.v.) blocked the inhibitory effect of CRF (1 microgram, i.c.v.), but the inhibitory effect of beta-endorphin (0.94 microgram, i.c.v.) was not changed by pretreatment with CRF-antiserum (1:20,000, 2.5 microliters, i.c.v.). These results suggest that PEA stimulates the release of hypothalamic CRF which stimulates the release of hypothalamic endogenous opioid peptides. The peptides again induces vagus nerve-mediated inhibitory effect on gastric acid sceretion.
本实验旨在研究2-吡啶乙胺（PEA，脑室内注射）对胃酸分泌抑制作用的中枢机制。通过灌注泵用37℃生理盐水持续冲洗雄性去肾上腺Wistar大鼠的胃酸。将药物注入第三脑室，以检测其对五肽胃泌素（160微克/千克，皮下注射）诱导的胃酸分泌的影响。结果如下：（1）用促肾上腺皮质激素释放因子抗血清（2.5微升，1:20,000，脑室内注射）预处理可阻断PEA（10微克，脑室内注射）对胃酸分泌的抑制作用。（2）注射促肾上腺皮质激素释放因子（1和2微克，脑室内注射）或β-内啡肽（0.94和1.25微克，脑室内注射）可显著抑制胃酸分泌。（3）用纳洛酮（5微克，脑室内注射）预处理可阻断促肾上腺皮质激素释放因子（1微克，脑室内注射）的抑制作用，但β-内啡肽（0.94微克，脑室内注射）的抑制作用不会因用促肾上腺皮质激素释放因子抗血清（1:20,000，2.5微升，脑室内注射）预处理而改变。这些结果表明，PEA刺激下丘脑促肾上腺皮质激素释放因子的释放，促肾上腺皮质激素释放因子又刺激下丘脑内源性阿片肽的释放。这些肽再次诱导迷走神经介导的对胃酸分泌的抑制作用。
