Li Y, Wang R, Lin S X, Jia B, Song K M
Department of Pathophysiology, Fourth Military Medical University, Xi'an.
Sheng Li Xue Bao. 1995 Jun;47(3):269-74.
In cultured vascular smooth muscle cells (VSMC) of SD rat, it was demonstrated that 10(-6) - 10(-5) mol/L NE and 10(-7) - 10(-5) mol/L isoproterenol stimulated proliferation and 3H-TdR incorporation of the cells in a dose dependent manner. These effects might be significantly inhibited by co-incubation with either alpha-receptor blocker phentolamine (10(-6) mol/L) or beta-receptor blocker propranolol (10(-5) mol/L). It was also found that cell counting and 3H-TdR incorporation were markedly decreased after incubation nifedipine (10(-6) mol/L) or verapamil (10(-6) mol/L) with NE (P < 0.01 as compared to that of NE group). It was further observed that nifedipine and verapamil inhibited DNA synthesis and proliferation of VSMC induced by isoproterenol.
在SD大鼠的培养血管平滑肌细胞（VSMC）中，已证明10（-6）-10（-5）mol/L的去甲肾上腺素（NE）和10（-7）-10（-5）mol/L的异丙肾上腺素以剂量依赖方式刺激细胞增殖和3H-胸腺嘧啶核苷（3H-TdR）掺入。与α受体阻滞剂酚妥拉明（10（-6）mol/L）或β受体阻滞剂普萘洛尔（10（-5）mol/L）共同孵育可显著抑制这些作用。还发现，硝苯地平（10（-6）mol/L）或维拉帕米（10（-6）mol/L）与NE孵育后，细胞计数和3H-TdR掺入明显减少（与NE组相比，P<0.01）。进一步观察到，硝苯地平和维拉帕米抑制异丙肾上腺素诱导的VSMC的DNA合成和增殖。
