Akai P S, Pungpak S, Chaicumpa W, Viroj K, Bunnag D, Befus A D
Department of Microbiology and Infectious Diseases, University of Calgary, Canada.
Trans R Soc Trop Med Hyg. 1994 Jul-Aug;88(4):471-4. doi: 10.1016/0035-9203(94)90438-3.
The liver flukes Opisthorchis viverrini and Clonorchis sinensis chronically infect over 30 million people in south-eastern Asia, resulting in significant morbidity and a predisposition to cholangiocarcinoma (CCA). Liver fluke-associated CCA carries a poor prognosis, partly because it is often detected at a late and advanced stage. The development of improved diagnostic methods, particularly for early CCA, may improve chances of survival and cure. Accordingly, we explored the use of immunological responses to liver fluke antigens as a potential means of identifying individuals at high risk for liver fluke-associated CCA. Serum antibody responses to O. viverrini adult worm homogenate and metacercaria homogenate (MH) were studied using enzyme-linked immunosorbent and immunoblot assays in 65 infected residents of an opisthorchiasis-endemic area in Thailand. Antibody levels correlated with liver ultrasonography (U/S) findings, and immunoblot analysis revealed a 91/93 kDa MH doublet recognized only by sera of individuals with severe liver U/S findings, including CCA. These results suggest that serum antibody responses to liver fluke antigens may be useful in the identification of infected individuals who are at high risk for liver fluke-associated CCA.
肝吸虫华支睾吸虫和中华肝吸虫长期感染东南亚地区超过3000万人，导致严重发病并易患胆管癌（CCA）。肝吸虫相关的CCA预后较差，部分原因是它常常在晚期和进展期才被发现。改进诊断方法，尤其是针对早期CCA的诊断方法，可能会提高生存和治愈的几率。因此，我们探索将对肝吸虫抗原的免疫反应作为识别肝吸虫相关CCA高危个体的一种潜在手段。在泰国一个华支睾吸虫病流行地区的65名受感染居民中，使用酶联免疫吸附测定和免疫印迹法研究了对华支睾吸虫成虫匀浆和后尾蚴匀浆（MH）的血清抗体反应。抗体水平与肝脏超声检查（U/S）结果相关，免疫印迹分析显示，只有包括CCA在内的肝脏U/S检查结果严重的个体血清能识别出91/93 kDa的MH双峰。这些结果表明，对肝吸虫抗原的血清抗体反应可能有助于识别肝吸虫相关CCA的高危感染个体。
