Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Int J Infect Dis. 2022 Mar;116:80-84. doi: 10.1016/j.ijid.2021.12.347. Epub 2021 Dec 23.
Opisthorchiasis is caused by an infection with fish-borne liver flukes of the genus Opisthorchis. Opisthorchiasis frequently leads to chronic inflammation in the biliary tract and is classified as a group 1 biological carcinogen by the International Agency for Research on Cancer: a definitive risk for cholangiocarcinoma (CCA).
We used the rapid immunochromatographic test (ICT) to detect anti-Opisthorchis viverrini IgG and IgG4 subclass antibodies in sera of patients with CCA. The ICT kits were developed based on soluble antigens excreted and secreted by O. viverrini adult worms.
ICT indicated sera was positive for IgG and IgG4 antibodies, respectively, in 22 (61.1%) and 15 (41.6%) participants of the 36 study participants diagnosed with CCA (P > 0.05). Our study also included groups with other cancers and with liver cirrhosis, where the IgG ICT and IgG4 ICT kits were 27.7% (13/47) and 25.5% (12/47) positive, respectively (P > 0.05). Neither total the IgG ICT nor the IgG4 ICT yielded positive results in a control group of 20 healthy participants. Moreover, the percentage positivity rate using the ICT for total IgG between the CCA group and the other cancers and liver cirrhosis group was significantly different (P < 0.05). By contrast, no significant difference between these groups was apparent in the ICT for IgG4 antibody. The CCA group was 6.53 times more likely to have positive anti-O. viverrini IgG antibody (odds ratio 6.53, P < 0.001) and 3.27 times more likely to have positive anti-O. viverrini IgG4 antibody (odds ratio 3.27, P = 0.010) than the non-CCA group.
This information is of potential value for the development of a diagnostic biomarker to predict risk for O. viverrini infection-associated CCA.
华支睾吸虫病是由鱼类源性肝吸虫属寄生虫感染引起的。华支睾吸虫病常导致胆道慢性炎症，并被国际癌症研究机构归类为第 1 组生物致癌物质：胆管癌（CCA）的明确风险。
我们使用快速免疫层析试验（ICT）检测 CCA 患者血清中的抗华支睾吸虫 IgG 和 IgG4 亚类抗体。ICT 试剂盒基于华支睾吸虫成虫排泄和分泌的可溶性抗原开发。
在 36 名被诊断为 CCA 的研究参与者中，有 22 名（61.1%）和 15 名（41.6%）参与者的 ICT 血清 IgG 和 IgG4 抗体呈阳性（P>0.05）。我们的研究还包括其他癌症和肝硬化组，其中 IgG ICT 和 IgG4 ICT 试剂盒的阳性率分别为 27.7%（13/47）和 25.5%（12/47）（P>0.05）。在 20 名健康对照组中，两种 IgG ICT 和 IgG4 ICT 试剂盒均未产生阳性结果。此外，ICT 用于总 IgG 的 CCA 组与其他癌症和肝硬化组之间的阳性率百分比差异具有统计学意义（P<0.05）。相比之下，这些组之间 IgG4 抗体的 ICT 没有明显差异。CCA 组发生阳性抗华支睾吸虫 IgG 抗体的可能性是无 CCA 组的 6.53 倍（优势比 6.53，P<0.001），发生阳性抗华支睾吸虫 IgG4 抗体的可能性是无 CCA 组的 3.27 倍（优势比 3.27，P=0.010）。
这些信息对于开发诊断生物标志物以预测华支睾吸虫感染相关 CCA 的风险具有潜在价值。
