使用聚丙烯酰胺凝胶等电聚焦法对“旧大陆”利什曼原虫物种进行酶分析。

The use of isoelectric focusing on polyacrylamide gel for the enzymatic analysis of 'Old World' Leishmania species.

作者信息

Piarroux R, Trouvé V, Pratlong F, Martini A, Lambert M, Rioux J A

机构信息

Laboratoire d'Ecologie Médicale et Pathologie Parasitaire, Faculté de Médecine, Université de Montpellier, France.

出版信息

Trans R Soc Trop Med Hyg. 1994 Jul-Aug;88(4):475-8. doi: 10.1016/0035-9203(94)90439-1.

DOI:10.1016/0035-9203(94)90439-1

PMID:7570849

Abstract

Using isoelectric focusing (IEF), a high resolution electrophoresis technique, we analysed 6 enzymes of 24 cloned strains representing all major taxa of 'Old World' Leishmania. The comparison of enzymatic patterns obtained with IEF and starch gel electrophoresis showed that IEF is a more discriminatory and more informative technique for the enzymatic analysis of Leishmania strains; it can detect very slight differences between 2 electromorphs not revealed with starch gel electrophoresis. Moreover, IEF detected several multi-banded patterns which appeared as single bands with starch gel electrophoresis. These multi-banded patterns could not be the result of strain heterogeneity since all the strains had been cloned. Their significance in the biology of Leishmania is discussed.

摘要

我们使用等电聚焦（IEF）这一高分辨率电泳技术，分析了代表“旧世界”利什曼原虫所有主要分类群的24个克隆菌株的6种酶。将IEF与淀粉凝胶电泳所得酶谱进行比较，结果表明，IEF在利什曼原虫菌株的酶分析中是一种更具区分性且信息更丰富的技术；它能够检测出淀粉凝胶电泳未显示的两个电变体之间非常细微的差异。此外，IEF检测到几种多带型模式，而在淀粉凝胶电泳中这些模式表现为单带。由于所有菌株均已克隆，这些多带型模式不可能是菌株异质性的结果。文中对它们在利什曼原虫生物学中的意义进行了讨论。