来自苏丹的利什曼原虫分离株的多样性：杜氏利什曼原虫的同工酶同质性与硕大利什曼原虫的异质性

Diversity among Leishmania isolates from the Sudan: isoenzyme homogeneity of L. donovani versus heterogeneity of L. major.

作者信息

Ibrahim M E, Evans D A, Theander T G, el Hassan A M, Kharazmi A

机构信息

Centre for Medical Parasitology, University of Copenhagen, Denmark.

出版信息

Trans R Soc Trop Med Hyg. 1995 Jul-Aug;89(4):366-9. doi: 10.1016/0035-9203(95)90010-1.

DOI:10.1016/0035-9203(95)90010-1

PMID:7570863

Abstract

Leishmania isolates from patients in the Sudan suffering from either visceral or cutaneous leishmaniasis were characterized using a battery of 12 enzymes. Aspartate aminotransferase separated the L. donovani isolates into 2 distinct zymodemes, but the overall results showed no significant geographical variation among L. donovani isolates. In contrast, the isolates of L. major were polymorphic, exhibiting differences in nucleoside hydrolase, 6-phosphogluconate dehydrogenase, superoxide dismutase, esterase, mannose phosphate isomerase, and aspartate aminotransferase, resulting in the description of 4 new enzymatic variants.

摘要

利用一组12种酶对来自苏丹患有内脏利什曼病或皮肤利什曼病患者的利什曼原虫分离株进行了表征。天冬氨酸转氨酶将杜氏利什曼原虫分离株分为2个不同的酶型，但总体结果显示杜氏利什曼原虫分离株之间没有显著的地理差异。相比之下，硕大利什曼原虫分离株具有多态性，在核苷水解酶、6-磷酸葡萄糖酸脱氢酶、超氧化物歧化酶、酯酶、甘露糖磷酸异构酶和天冬氨酸转氨酶方面存在差异，从而描述了4种新的酶变体。