Tanaka S, Kamiike W, Ito T, Uchikoshi F, Matsuda H, Nozawa M, Kumura E, Shiga T, Kosaka H
First Department of Surgery, Osaka University Medical School, Japan.
Transplantation. 1995 Oct 15;60(7):713-7. doi: 10.1097/00007890-199510150-00017.
In clinical pancreas transplantation, no reliable marker for the early diagnosis of acute rejection has been reported. This is one reason why the graft survival rate of pancreas transplantation alone is much lower than that of other organs, such as hearts, livers, and kidneys. We designed an experiment to investigate acute rejection of pancreas allografts in hyperglycemic rats by measurement of blood glucose levels and nitric oxide (NO) products (nitrite plus nitrate, and nitrosyl hemoglobin). As recipients, Lewis rats were rendered hyperglycemic by intravenous injection of streptozotocin before transplantation. F344 rats were used as donors of pancreas allografts. Lewis rats were also used as donors of syngeneic pancreas grafts. After transplantation, the blood glucose level returned to a normal level and rejection was defined as the recurrence of hyperglycemia. The mean survival time of pancreas allografts was 14 +/- 0.7 days. The plasma level of nitrite plus nitrate in allografted rats peaked on postoperative day 7. Electron spin resonance spectra of NO bound to hemoglobin were detected in the blood from allografted rats with a peak on postoperative day 7, whereas NO bound to hemoglobin was not detected in the blood from recipients of syngeneic grafts at any sampling time. The results show that NO was synthesized in the earlier period than the elevation of the blood glucose level during rejection after pancreas transplantation in rats.
在临床胰腺移植中，尚未有关于急性排斥反应早期诊断的可靠标志物的报道。这就是单纯胰腺移植的移植物存活率远低于心脏、肝脏和肾脏等其他器官的原因之一。我们设计了一项实验，通过测量血糖水平和一氧化氮（NO）产物（亚硝酸盐加硝酸盐以及亚硝基血红蛋白）来研究高血糖大鼠胰腺同种异体移植物的急性排斥反应。作为受体，Lewis大鼠在移植前通过静脉注射链脲佐菌素使其血糖升高。F344大鼠用作胰腺同种异体移植物的供体。Lewis大鼠也用作同基因胰腺移植物的供体。移植后，血糖水平恢复到正常水平，排斥反应定义为高血糖复发。胰腺同种异体移植物的平均存活时间为14±0.7天。同种异体移植大鼠中亚硝酸盐加硝酸盐的血浆水平在术后第7天达到峰值。在同种异体移植大鼠的血液中检测到与血红蛋白结合的NO的电子自旋共振光谱，其峰值出现在术后第7天，而在任何采样时间，同基因移植物受体的血液中均未检测到与血红蛋白结合的NO。结果表明，在大鼠胰腺移植排斥反应过程中，NO的合成早于血糖水平升高的时间。
