The effect of refraction and assumed speeds of sound in tissue and blood on Doppler ultrasound blood velocity measurements.

The combined effect of three assumptions relating to refraction, the speed of sound in tissue and the speed of sound in blood on the accuracy of Doppler ultrasound blood velocity measurements has been investigated. A theoretical relationship giving the net velocity measurement error introduced by these three assumptions has derived using a model in which tissue and blood layers are separated by straight, parallel boundaries. This net error is dependent on the assumed and actual speed of sound in tissue, the assumed speed of sound in blood and the Doppler angle, but is effectively independent of the actual speed of sound in blood. For clinical blood velocity measurements, the net error is estimated to be as much as an 8% overestimation of the actual velocity, higher than previously predicted for any of the factors individually. The relationship also predicts a net velocity measurement error in experimental flow systems and string phantoms which is dependent on the speed of sound in the liquid bath. A water bath at room temperature will give an overestimation of approximately 2%. Experimental investigations using conventional and modified string phantoms and a 5-MHz linear phased array system support these conclusions. The effect of perturbing the layers from their parallel orientation has also been considered theoretically and has provided additional support for the above conclusions. These results may help more accurate Doppler velocity measurements in both experimental and clinical settings.