van Duijkeren E, Flemming C, Sloet van Oldruitenborgh-Oosterbaan M, Kalsbeek H C, van der Giessen J W
Department of Large Animal Medicine and Nutrition, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
Vet Q. 1995 Jun;17(2):63-6. doi: 10.1080/01652176.1995.9694534.
Three rectal faecal samples were taken at 24-hour intervals from 136 horses in order to investigate whether multiple faecal cultures yield a greater number of Salmonella-positive horses compared to single faecal cultures. Of these 136 horses, 89 were suspected of salmonellosis on clinical grounds and 47 belonged to a control group. From the 'Salmonella suspected' group, 22 horses (25%) were Salmonella positive on one or more occasions. Only twelve of these 22 positive horses (55%) were positive at first sampling. Of the control group, only three horses (6%) were positive for Salmonella. Thirty-one (69%) of the 45 positive cultures from the 'Salmonella suspected' group were found only after enrichment in Selenite broth. From the results of the present study it is concluded that multiple faecal cultures are superior to single faecal cultures for diagnosing clinical salmonellosis in horses and that there is no relation between the course of the disease and whether the Salmonellae were cultured directly or indirectly, after enrichment in Selenite broth. Salmonellae were cultured from the faeces of horses treated with antibiotics before their referral, even when the isolated strain was sensitive to the antimicrobial drug used by the practitioner.
为了研究与单次粪便培养相比，多次粪便培养是否能检测出更多沙门氏菌呈阳性的马匹，每隔24小时从136匹马采集三份直肠粪便样本。在这136匹马中，89匹因临床症状怀疑患有沙门氏菌病，47匹属于对照组。在“疑似沙门氏菌”组中，22匹马（25%）在一次或多次检测中沙门氏菌呈阳性。这22匹阳性马中只有12匹（55%）在首次采样时呈阳性。对照组中，只有3匹马（6%）沙门氏菌呈阳性。“疑似沙门氏菌”组的45份阳性培养物中，有31份（69%）是在亚硒酸盐肉汤中富集后才检测到的。从本研究结果可以得出结论，多次粪便培养在诊断马匹临床沙门氏菌病方面优于单次粪便培养，并且疾病进程与沙门氏菌是直接培养还是在亚硒酸盐肉汤中富集后间接培养无关。在转诊前用抗生素治疗过的马匹粪便中也培养出了沙门氏菌，即使分离出的菌株对从业者使用的抗菌药物敏感。
