Cohen N D, Neibergs H L, Wallis D E, Simpson R B, McGruder E D, Hargis B M
Department of Large Animal Medicine & Surgery, College of Veterinary Medicine, Texas A&M University, College Station 77843.
Am J Vet Res. 1994 Aug;55(8):1049-54.
Members of the genus Salmonella were identified in feces from horses, using the polymerase chain reaction (PCR) and genus-specific oligonucleotide primers. Feces from healthy horses were determined to be culture-negative for Salmonella spp. Fecal samples were inoculated with known numbers of colony-forming units (CFU) of S anatum, S derby, S enteritidis, S heidelberg, S newport, and S typhimurium. The DNA was extracted from fecal samples and amplified by PCR, using genus-specific primers. Sensitivity of the assay extended to 10(3) CFU of Salmonella sp/g of feces; sensitivity of microbiologic culture with enrichment extended to 10(2) CFU of Salmonella sp/g of feces. Feces that were not inoculated with Salmonella spp were negative by the PCR. Detection of Salmonellae in feces was possible, using the PCR, within 10 to 12 hours from the time of submission of samples.
利用聚合酶链反应（PCR）和属特异性寡核苷酸引物，在马的粪便中鉴定出沙门氏菌属成员。经测定，健康马的粪便中沙门氏菌属培养呈阴性。将粪便样本接种已知数量的肠炎沙门氏菌、德比沙门氏菌、肠炎沙门氏菌、海德堡沙门氏菌、纽波特沙门氏菌和鼠伤寒沙门氏菌的菌落形成单位（CFU）。从粪便样本中提取DNA，并使用属特异性引物通过PCR进行扩增。该检测方法的灵敏度可达到每克粪便10³CFU的沙门氏菌；富集微生物培养的灵敏度可达到每克粪便10²CFU的沙门氏菌。未接种沙门氏菌属的粪便经PCR检测呈阴性。从提交样本之时起，利用PCR在10至12小时内即可检测出粪便中的沙门氏菌。
