Adúriz J J, Juste R A, Cortabarria N
Servicio de Investigación y Mejora Agraria (SIMA), Dpto. de Agricultura y Pesca, Derio, Vizcaya, Spain.
Vet Microbiol. 1995 Jul;45(2-3):211-7. doi: 10.1016/0378-1135(95)00037-b.
Mycobacterium avium subsp. paratuberculosis is distinguished from other mycobacterial species by its dependence on the iron-chelator factor mycobactin and its prolonged incubation period when grown in vitro. Traditionally, a very low rate of isolation has been considered characteristic of sheep strains of this mycobacterium species. In the present study, a comparison was made of the performance of two media, Löwenstein-Jensen and Middlebrook 7H11/OADC both with and without mycobactin, on the isolation of M. a. paratuberculosis from ovine cases of the disease. A high isolation rate in both media was observed. Moreover, our results indicated that mycobactin dependence of sheep strains of M. a. paratuberculosis is medium related, being dependent on Löwenstein-Jensen and independent on Middlebrook 7H11/OADC.
副结核分枝杆菌鸟亚种与其他分枝杆菌物种的区别在于其对铁螯合剂因子分枝杆菌素的依赖性以及在体外生长时较长的潜伏期。传统上，这种分枝杆菌物种的绵羊菌株的分离率一直被认为非常低。在本研究中，对两种培养基（含和不含分枝杆菌素的罗-琴培养基和Middlebrook 7H11/OADC培养基）从该病的绵羊病例中分离副结核分枝杆菌鸟亚种的性能进行了比较。在两种培养基中均观察到高分离率。此外，我们的结果表明，副结核分枝杆菌鸟亚种绵羊菌株对分枝杆菌素的依赖性与培养基有关，依赖于罗-琴培养基，而不依赖于Middlebrook 7H11/OADC培养基。
