Mercer A A, Lyttle D J, Whelan E M, Fleming S B, Sullivan J T
Health Research Council Virus Research Unit, University of Otago, Dunedin, New Zealand.
Virology. 1995 Oct 1;212(2):698-704. doi: 10.1006/viro.1995.1527.
The large differences between the G+C content of the orf virus genome and those of other characterized poxviruses have precluded the use of DNA hybridization to establish a gene map of orf virus. Here we have sequenced the ends of cloned restriction endonuclease fragments of the nZ2 strain of orf virus (OV) and used the translated sequences to search protein data bases. Sequence from 15 points found high-scoring matches to data base entries, including 18 vaccinia virus (VAC) genes. We also present 2 kb of sequence from a region near the right terminus of the OV genome and show that it encodes homologs of VAC genes, F9L and F10L. The data presented here in conjunction with published and as yet unpublished data have allowed the construction of a gene map of OV on which 37 genes have been placed. Thirty-two of these genes have homologs in VAC. Alignment of the OV gene map with that of VAC revealed that each OV gene and its VAC counterpart occurred in the same order and orientation on their respective genomes. The intervals between many of the points of sequence were also found to be strikingly similar. The conserved spacing of genes between OV and VAC within the central 88.2 kb of the 139-kb OV genome is not maintained in the termini where insertion, deletion, and translocation have occurred. Parallels are drawn between the data presented here and related data from swinepox virus and capripox virus.
口疮病毒基因组的G+C含量与其他已鉴定的痘病毒之间存在巨大差异，这使得无法利用DNA杂交技术来构建口疮病毒的基因图谱。在此，我们对口疮病毒（OV）nZ2株克隆的限制性内切酶片段的末端进行了测序，并利用翻译后的序列搜索蛋白质数据库。从15个位点获得的序列与数据库条目有高分匹配，其中包括18个痘苗病毒（VAC）基因。我们还展示了来自OV基因组右末端附近一个区域的2 kb序列，并表明它编码VAC基因F9L和F10L的同源物。本文提供的数据与已发表和尚未发表的数据相结合，使得构建出了一张OV基因图谱，上面已标注了37个基因。其中32个基因在VAC中有同源物。将OV基因图谱与VAC的基因图谱进行比对后发现，每个OV基因及其VAC对应基因在各自基因组上的排列顺序和方向相同。还发现许多序列位点之间的间隔也惊人地相似。在139 kb的OV基因组中央88.2 kb区域内，OV和VAC之间基因的保守间距在末端并未保持，末端发生了插入、缺失和易位。本文提供的数据与猪痘病毒和山羊痘病毒的相关数据进行了比较。
