Wang H B, Chen W Z, Bao E J, Zheng Q Y, Song H L, Fang J, Xu Y X, Chen H S
Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai.
Yao Xue Xue Bao. 1995;30(6):401-7.
Phytolacca acinosa polysaccharides I (PAP-I), a kind of purified polysaccharides, isolated from Phytolacca acinosa Roxb was found to significantly augment the cytotoxicity of murine splenocytes and interleukin-2 (IL-2) activated splenocytes against P815 tumor cells in vitro. The optimal concentration of PAP-I was 1 microgram.ml-1 and the peak level of the cytotoxicity against P815 tumor cells was reached on d 3-5. The supernatants collected from splenocytes cultured with PAP-I alone or in combination with IL-2 showed no effect on the cytotoxicity against P815 tumor cells. Splenocytes from mice injected ip with PAP-I, 5, 10 and 50 mg.kg-1, thrice a week produced more cytotoxicity against P815 and L929 tumor cells compared with the control group. PAP-I ip was shown to significantly increase IL-2 activated killer cell activity (LAK) against P815 tumor cells. The higher the dosage of PAP-I, the more potent the LAK activity was observed. These results confirmed that PAP-I can augment the cytotoxicity of murine splenocyte against tumor cells and LAK activity and warranted further evaluation of its clinical usefulness.
商陆多糖I（PAP-I）是从商陆中分离得到的一种纯化多糖，体外实验发现其可显著增强小鼠脾细胞及白细胞介素-2（IL-2）激活的脾细胞对P815肿瘤细胞的细胞毒性。PAP-I的最佳浓度为1微克/毫升，在第3至5天达到对P815肿瘤细胞的细胞毒性峰值。单独用PAP-I或与IL-2联合培养脾细胞收集的上清液对P815肿瘤细胞的细胞毒性无影响。每周三次腹腔注射5、10和50毫克/千克体重PAP-I的小鼠脾细胞，与对照组相比，对P815和L929肿瘤细胞产生更强的细胞毒性。腹腔注射PAP-I可显著增强对P815肿瘤细胞的IL-2激活杀伤细胞活性（LAK）。PAP-I剂量越高，观察到的LAK活性越强。这些结果证实PAP-I可增强小鼠脾细胞对肿瘤细胞的细胞毒性及LAK活性，值得进一步评估其临床应用价值。
