Wang H B, Zheng Q Y, Ju D W, Fang J
Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai.
Yao Xue Xue Bao. 1993;28(10):732-7.
The effects of Phytolacca acinosa polysaccharides I (PAP-I), a polysaccharide extracted from Phytolacca acinosa Roxb: on splenic lymphocyte proliferation and cytokines production from splenic lymphocyte and macrophage were studied. Lipopolysaccharides (LPS) and PAP-I were found to significantly augment splenic lymphocyte proliferation of normal BALB/c, nude BALB/c and NC mice in vitro, but concanavalin A (Con A) was shown to stimulate only normal BALB/c and nude BALB/c splenic lymphocyte proliferation. Also, PAP-I significantly enhanced Con A or LPS-induced lymphocyte proliferation and mixed lymphocyte reaction. Significant enhancement of colony stimulating factor (CSF) production was observed from splenic lymphocyte of normal BALB/c and nude BALB/c mice but not from NC mice when treated with PAP-I for 5 d. PAP-I was shown to significantly enhance interleukin-2 (IL-2) production from normal mice splenocyte and Con A stimulated normal mice splenocyte in a concentration-dependent fashion. Supernatant of PAP-treated macrophage (M phi) were collected and CSF activity was tested. The results confirmed that PAP-I can significantly stimulate M phi to secret CSF activity on d 1. The supernatant also contained a cytokine which exhibited a synergistic action with recombinant murine granular-macrophage CSF (RMGM-CSF) to stimulate mice bone marrow cell proliferation. PAP-I, 5-50 mg.kg-1, ip can enhanced splenic lymphocyte proliferation and IL-2 production. These findings indicate that PAP-I can augment immunologic function in vitro and in vivo.
研究了从商陆中提取的一种多糖——商陆多糖I（PAP-I）对脾淋巴细胞增殖以及脾淋巴细胞和巨噬细胞产生细胞因子的影响。结果发现，脂多糖（LPS）和PAP-I在体外能显著增强正常BALB/c、裸BALB/c和NC小鼠的脾淋巴细胞增殖，但伴刀豆球蛋白A（Con A）仅能刺激正常BALB/c和裸BALB/c小鼠的脾淋巴细胞增殖。此外，PAP-I显著增强Con A或LPS诱导的淋巴细胞增殖及混合淋巴细胞反应。用PAP-I处理5天时，观察到正常BALB/c和裸BALB/c小鼠的脾淋巴细胞中集落刺激因子（CSF）产生显著增强，而NC小鼠未出现这种情况。PAP-I能以浓度依赖的方式显著增强正常小鼠脾细胞以及Con A刺激的正常小鼠脾细胞产生白细胞介素-2（IL-2）。收集PAP处理的巨噬细胞（M phi）的上清液并检测CSF活性。结果证实，PAP-I在第1天能显著刺激M phi分泌CSF活性。上清液中还含有一种细胞因子，它与重组小鼠粒-巨噬细胞集落刺激因子（RMGM-CSF）协同作用，刺激小鼠骨髓细胞增殖。腹腔注射5 - 50 mg·kg-1的PAP-I可增强脾淋巴细胞增殖和IL-2产生。这些发现表明，PAP-I在体外和体内均能增强免疫功能。
